CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a revolutionary gene-editing technology derived from a natural bacterial immune system.
Key points:
Uses a guide RNA to direct the Cas9 protein to cut specific DNA sequences.
Allows precise insertion, deletion, or modification of genes.
Applications: curing genetic diseases, improving crops, biomedical research.
Nobel Prize in Chemistry (2020) awarded to Doudna & Charpentier for its discovery.
It’s faster, cheaper, and more accurate than older gene-editing methods.
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CRISPRSingle and Systems-Level Multi-Omics Integration: Building a Closed-Loop System for Functional Genomics 1. Technical Framework and Core Logic CRISPRSingle refers to a technology integrating CRISPR gene editing at the single-cell level with multi-omics analysis. Its core objective is to establi…
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CRISPR + Single-Cell Sequencing: A Deep Dive into Technical Integration 1. Definition and Core Principles CRISPR + Single-Cell Sequencing is an innovative approach that combines CRISPR gene-editing technology with single-cell resolution omics analysis (e.g., single-cell RNA sequencing). Its goal is …
CRISPR-Toolkit: Definition, Core Components, and Applications Definition and Conceptual Scope CRISPR-Toolkit refers to a collection of software, experimental reagents, and standardized protocols developed around CRISPR gene-editing technology. It aims to simplify the complexity of genetic editing wh…
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1. Definition and Core Mechanisms CRISPR Target refers to the DNA or RNA sequences specifically recognized and cleaved by CRISPR systems (e.g., Cas9, Cas13 nucleases) guided by RNA (gRNA or crRNA). This process underpins CRISPR’s ability to achieve gene editing, antiviral immunity, or diagnostic fun…
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