CRISPRSingle and Systems-Level Multi-Omics Integration: Building a Closed-Loop System for Functional Genomics 1. Technical Framework and Core Logic CRISPRSingle refers to a technology integrating CRISPR gene editing at the single-cell level with multi-omics analysis. Its core objective is to establi…
Genetic Technologies and Human Longevity: Breakthroughs, Ethical Challenges, and Future Visions 1. Core Mechanisms of Lifespan Extension via Genetic Technologies Precision Regulation of Aging-Related Genes Gene Repair: CRISPR-Cas9 tools correct progeria-causing mutations (e.g., LMNA) or inhibit pro-…
编辑组学：定义、学科范围和未来应用 定义和学科范围 Editomics 是“Edit”和“Omics”的合成词，代表了一个科学领域，专注于通过将基因编辑技术与多组学方法（基因组学、转录组学、蛋白质组学等）相结合来系统分析生物编辑事件。其研究范围包括： 基因组编辑组学：使用 CRISPR、碱基编辑等对基因组进行靶向修饰，以研究系统性的生物学影响。 RNA 编辑组学：研究 RNA 水平编辑事件（例如，ADAR 介导的 A-to-I 编辑）及其在疾病中的作用，例如 EDK 数据库中记录的异常 RNA 编辑-疾病关联。 表观遗传编辑组学：调节表观遗传标记（例如，DNA 甲基化、组蛋白修饰）以改变基因表…
CRISPR + Single-Cell Sequencing: A Deep Dive into Technical Integration 1. Definition and Core Principles CRISPR + Single-Cell Sequencing is an innovative approach that combines CRISPR gene-editing technology with single-cell resolution omics analysis (e.g., single-cell RNA sequencing). Its goal is …
CRISPR-Toolkit: Definition, Core Components, and Applications Definition and Conceptual Scope CRISPR-Toolkit refers to a collection of software, experimental reagents, and standardized protocols developed around CRISPR gene-editing technology. It aims to simplify the complexity of genetic editing wh…
1. Definition and Core Mechanisms CRISPR Target refers to the DNA or RNA sequences specifically recognized and cleaved by CRISPR systems (e.g., Cas9, Cas13 nucleases) guided by RNA (gRNA or crRNA). This process underpins CRISPR’s ability to achieve gene editing, antiviral immunity, or diagnostic fun…